Depo® Estradiol Cypionate Injection, USP

(Commercial Product)

Available Dosage Strengths

5 mg/mL

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Disclaimer: Images are for reference only; actual products may vary.

Product Overview

Description

Estradiol Cypionate Injection, USP Description^1,2,3

Estradiol cypionate is a long-acting injectable ester of 17β-estradiol, the principal estrogen produced by the ovaries in premenopausal women. It is formulated for intramuscular (IM) injection, typically in oil-based solutions allowing for slow release and prolonged systemic activity.

Mechanism of Action

Mechanism of Action^1,2,3

Estradiol binds to estrogen receptors, which are widely distributed in reproductive tissues, the brain, bone, liver, and vasculature. Upon receptor binding, estradiol influences gene transcription, which may lead to a broad range of systemic and local effects:

Endometrial proliferation in the absence of a progesterone or progestogen.
Negative feedback on the hypothalamic-pituitary axis (reduces FSH and LH)
Maintenance of bone density via inhibition of bone resorption
Thermoregulatory stabilization, reducing hot flashes and night sweats
Cardiovascular and lipid effects (e.g., improves HDL/LDL ratio)
Neurotransmitter modulation affecting mood and cognition

Adverse Reactions

Common^1,2,3

Breast tenderness
Nausea
Headache
Fluid retention
Bloating
Mood changes
Breakthrough vaginal bleeding or spotting
Injection site discomfort

Contraindications & Precautions

Absolute Contraindications^1,2,3

Known or suspected breast cancer or estrogen-dependent neoplasia
Undiagnosed abnormal genital bleeding
Active or history of thromboembolic disorders (e.g., DVT, PE, stroke)
Active liver dysfunction or disease
Known hypersensitivity to estradiol cypionate or its formulation components (e.g., sesame oil)

Precautions^1,2,3

Women with a history of hormone-sensitive cancers
Hypertension or cardiovascular disease
Migraine with aura
Diabetes mellitus
Hypertriglyceridemia
In women with an intact uterus, a progestogen must be co-administered to reduce the risk of endometrial hyperplasia and cancer.
Routine monitoring of breast, pelvic, and cardiovascular health is advised during therapy.

Storage

Store at controlled room temperature (20°C to 25°C / 68°F to 77°F)
Protect from moisture and light
Keep in a tightly closed container

References

Clinical Pharmacology (Elsevier) – Estradiol Cypionate Monograph; accessed August 2025 through: https://www.clinicalkey.com/pharmacology/monograph/1330?n=Estradiol
FDA Label – Depo-Estradiol (estradiol cypionate injection); accessed August 2025 through: https://www.accessdata.fda.gov/drugsatfda_docs/label/2017/009747s037lbl.pdf
MedlinePlus – Estradiol Injection (systemic) accessed August 2025 through: https://medlineplus.gov/druginfo/meds/a682750.html

Disclaimer

Wells Pharmacy Network distributes certain FDA-approved commercial products that are manufactured by third-party pharmaceutical companies. These products are not compounded or manufactured by Wells Pharmacy Network. Product availability at the time of dispensing may vary due to supply chain conditions.

This information is for educational purposes only. Compounded products have not been reviewed, approved, or evaluated by the FDA for safety and effectiveness. It is not intended to diagnose, treat, cure, or prevent any disease. No claims are made regarding treatment, cure, or prevention of any disease or condition.

A Wells Pharmacy Network pharmacist is available to answer any questions. For consultation, please call 1-800-622-4510.